Part:BBa_K3487007
Lysine riboswitch
Description
Riboswitch is a regulatory part of an mRNA molecule that can specifically bind a metabolite and regulate gene expression. Till now, more than 20 riboswitches have been discovered. Among them, only two amino acids riboswitches were found: a lysine riboswitch and a glycine riboswitch. Several previous studies examined the functionality and manipulation of the B. subtilis lysine riboswitch. A lysine riboswitch was also found in E. coli, which revealed in fact the mechanism of lysine feedback repression of aspartkinase III in E. coli. The following is the control principle diagram
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 12
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 284
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 256
2020 SZPT-CHINA
Result
In our project, we set up a suicide mechanism regulated by this lysine riboswitch part. When the bacteria colonize the oral cavity, the riboswitch controlled by lysine is turned off. When the bacteria escape the oral environment, the riboswitch opens and expresses the toxic protein RalR, which is a non-specific endonuclease. In order to determine at what concentration of lysine this switch can be turned on. We connected sfGFP to lysine riboswitch and then tested the fluorescent expression in different concentrations of lysine for 24 hours. The result is as follows.
References
Garst AD, Héroux A, Rambo RP, Batey RT. Crystal structure of the lysine riboswitch regulatory mRNA element. J Biol Chem. 2008;283(33):22347-22351.
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